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horseradish peroxidase coupled avidin biotin complex  (Vector Laboratories)


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    Structured Review

    Vector Laboratories horseradish peroxidase coupled avidin biotin complex
    Horseradish Peroxidase Coupled Avidin Biotin Complex, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 12897 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/avidin+biotin+horseradish+peroxidase+kit/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/10__1016_slash_j__bramec__2026__202560-63-1-5
    Average 99 stars, based on 12897 article reviews
    horseradish peroxidase coupled avidin biotin complex - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Early survival and delayed death of developmentally-born dentate gyrus neurons.
    Article Snippet: For stereological quantification of BrdU1 cells, a 1 in 12 series of sections throughout the entire DG were mounted onto slides, heated to 90 8C in citric acid (0.1 M, pH 6.0), permeabilized in PBS with 10% Triton-X for 30 min and then incubated overnight with goat anti-Prox1 (1:1,000 in 10% Triton-X and 3% horse serum; R&D Systems, Minneapolis, MN, AF2727). .. Sections were washed, incubated in biotinylated donkey anti-goat secondary antibody for 1 hr (1:250; Jackson, West Grove, PA, 705-065-147), 0.3% hydrogen peroxide for 30 min and Prox11 cells were visualized with an avidin-biotin-horseradish peroxidase kit (Vector Laboratories , Burlingame, CA) and vector nova red HRP substrate (Vector Laboratories). .. Sections were washed, permeabilized with trypsin, incubated in 2 N HCl for 30 min to denature DNA, and incubated overnight with mouse anti-BrdU (1:200 in 10% Triton-X and 3% horse serum, BD Biosciences, San Jose, CA 347580).

    Article Title: Fluoxetine Pretreatment Promotes Neuronal Survival and Maturation after Auditory Fear Conditioning in the Rat Amygdala
    Article Snippet: .. Sections were incubated for 15 min in 3% hydrogen peroxide to eliminate endogenous peroxidases, then rinsed and incubated with blocking buffer (5% bovine serum albumin, 0.3% Triton X-100) for 1 h. Sections were then incubated with a mouse anti-BrdU antibody (1∶1000; Sigma) overnight at 4°C, rinsed and transferred to biotinylated goat anti-mouse IgG (1∶200; Vector Laboratories) at room temperature for 2 h. BrdU was visualized using an avidin-biotin-horseradish peroxidase kit (Vector Laboratories) and reacted with diaminobenzidine (Sigma). ..

    Article Title: Differential Effects of Extended Exercise and Memantine Treatment on Adult Neurogenesis in Male and Female Rats.
    Article Snippet: .. Sections were washed and incubated in biotinylated goat anti-mouse (Sigma, cat #B0529) or biotinylated donkey anti-rat (Jackson, cat #712065153) secondary antibody for 1 hour (1:250), tissues was blocked in 0.3% H2O2 for 30 min, and cells were then visualized with an avidin-biotin-horseradish peroxidase kit (Vector Laboratories, cat #OK-6100) and cobaltenhanced DAB (Sigma Fast Tablets, cat #DO426). ..

    Article Title: Quantification and correlation of amyloid-β plaque load, glial activation, GABAergic interneuron numbers, and cognitive decline in the young TgF344-AD rat model of Alzheimer’s disease
    Article Snippet: .. After thorough rinsing, the sections were incubated with a corresponding biotinylated secondary antibody for 2 h and labeling was visualized using an avidin-biotin-horseradish peroxidase kit (1:200; Vectastaine Elite ABC Kit, Vector Laboratories), and developed with diaminobenzidine (1:200; DAB Peroxidase Substrate Kit, Vector Laboratories). ..

    Article Title: Late Maturation of Adult-Born Neurons in the Temporal Dentate Gyrus
    Article Snippet: .. Sections were then incubated with mouse anti-BrdU antibody (1∶100; BD Biosciences) at 4°C overnight followed by biotinylated goat anti-mouse IgG (1∶200; Sigma) at room temperature for 1 h. BrdU was then visualized using an avidin-biotin-horseradish peroxidase kit (Vector Laboratories) and cobalt-enhanced DAB (Sigma Fast tablets). ..

    Article Title: Differential Effects of Extended Exercise and Memantine Treatment on Adult Neurogenesis in Male and Female Rats
    Article Snippet: .. Sections were washed and incubated in biotinylated goat anti-mouse (Sigma, cat #B0529) or biotinylated donkey anti-rat (Jackson, cat #712065153) secondary antibody for 1 hour (1:250), tissues was blocked in 0.3% H 2 O 2 for 30 min, and cells were then visualized with an avidin-biotin-horseradish peroxidase kit (Vector Laboratories, cat #OK-6100) and cobalt-enhanced DAB (Sigma Fast Tablets, cat #DO426). ..

    Avidin-Biotin Assay:

    Article Title: Early survival and delayed death of developmentally-born dentate gyrus neurons.
    Article Snippet: For stereological quantification of BrdU1 cells, a 1 in 12 series of sections throughout the entire DG were mounted onto slides, heated to 90 8C in citric acid (0.1 M, pH 6.0), permeabilized in PBS with 10% Triton-X for 30 min and then incubated overnight with goat anti-Prox1 (1:1,000 in 10% Triton-X and 3% horse serum; R&D Systems, Minneapolis, MN, AF2727). .. Sections were washed, incubated in biotinylated donkey anti-goat secondary antibody for 1 hr (1:250; Jackson, West Grove, PA, 705-065-147), 0.3% hydrogen peroxide for 30 min and Prox11 cells were visualized with an avidin-biotin-horseradish peroxidase kit (Vector Laboratories , Burlingame, CA) and vector nova red HRP substrate (Vector Laboratories). .. Sections were washed, permeabilized with trypsin, incubated in 2 N HCl for 30 min to denature DNA, and incubated overnight with mouse anti-BrdU (1:200 in 10% Triton-X and 3% horse serum, BD Biosciences, San Jose, CA 347580).

    Article Title: Fluoxetine Pretreatment Promotes Neuronal Survival and Maturation after Auditory Fear Conditioning in the Rat Amygdala
    Article Snippet: .. Sections were incubated for 15 min in 3% hydrogen peroxide to eliminate endogenous peroxidases, then rinsed and incubated with blocking buffer (5% bovine serum albumin, 0.3% Triton X-100) for 1 h. Sections were then incubated with a mouse anti-BrdU antibody (1∶1000; Sigma) overnight at 4°C, rinsed and transferred to biotinylated goat anti-mouse IgG (1∶200; Vector Laboratories) at room temperature for 2 h. BrdU was visualized using an avidin-biotin-horseradish peroxidase kit (Vector Laboratories) and reacted with diaminobenzidine (Sigma). ..

    Article Title: A combination of running and memantine increases neurogenesis and reduces activation of developmentally-born dentate granule neurons in rats
    Article Snippet: .. Cells were then visualized with an avidin-biotin-horseradish peroxidase kit (Vector Laboratories, cat #OK-6100) and cobalt-enhanced 3,3’-diaminobenzidine (DAB) (Sigma Fast Tablets, cat #DO426). ..

    Article Title: Differential Effects of Extended Exercise and Memantine Treatment on Adult Neurogenesis in Male and Female Rats.
    Article Snippet: .. Sections were washed and incubated in biotinylated goat anti-mouse (Sigma, cat #B0529) or biotinylated donkey anti-rat (Jackson, cat #712065153) secondary antibody for 1 hour (1:250), tissues was blocked in 0.3% H2O2 for 30 min, and cells were then visualized with an avidin-biotin-horseradish peroxidase kit (Vector Laboratories, cat #OK-6100) and cobaltenhanced DAB (Sigma Fast Tablets, cat #DO426). ..

    Article Title: Elevated Interleukin-17A expression in amlodipine-induced gingival overgrowth.
    Article Snippet: © 2020 John Wiley & Sons A/S.. Published by John Wiley & Sons Ltd 1Department of Periodontology, Faculty of Dentistry, Kirikkale University, Kirikkale, Turkey 2Department of Periodontology, Faculty of Dentistry, Hacettepe University, Ankara, Turkey 3Department of Oral Pathology, Faculty of Dentistry, Gazi University, Ankara, Turkey 4Department of Periodontology, Faculty of Dentistry, Istanbul Aydin University, Istanbul, Turkey 5Department of Pediatric Immunology, Faculty of Medicine, Hacettepe University, Ankara, Turkey

    Article Title: Quantification and correlation of amyloid-β plaque load, glial activation, GABAergic interneuron numbers, and cognitive decline in the young TgF344-AD rat model of Alzheimer’s disease
    Article Snippet: .. After thorough rinsing, the sections were incubated with a corresponding biotinylated secondary antibody for 2 h and labeling was visualized using an avidin-biotin-horseradish peroxidase kit (1:200; Vectastaine Elite ABC Kit, Vector Laboratories), and developed with diaminobenzidine (1:200; DAB Peroxidase Substrate Kit, Vector Laboratories). ..

    Article Title: Late Maturation of Adult-Born Neurons in the Temporal Dentate Gyrus
    Article Snippet: .. Sections were then incubated with mouse anti-BrdU antibody (1∶100; BD Biosciences) at 4°C overnight followed by biotinylated goat anti-mouse IgG (1∶200; Sigma) at room temperature for 1 h. BrdU was then visualized using an avidin-biotin-horseradish peroxidase kit (Vector Laboratories) and cobalt-enhanced DAB (Sigma Fast tablets). ..

    Article Title: Differential Effects of Extended Exercise and Memantine Treatment on Adult Neurogenesis in Male and Female Rats
    Article Snippet: .. Sections were washed and incubated in biotinylated goat anti-mouse (Sigma, cat #B0529) or biotinylated donkey anti-rat (Jackson, cat #712065153) secondary antibody for 1 hour (1:250), tissues was blocked in 0.3% H 2 O 2 for 30 min, and cells were then visualized with an avidin-biotin-horseradish peroxidase kit (Vector Laboratories, cat #OK-6100) and cobalt-enhanced DAB (Sigma Fast Tablets, cat #DO426). ..

    Blocking Assay:

    Article Title: Fluoxetine Pretreatment Promotes Neuronal Survival and Maturation after Auditory Fear Conditioning in the Rat Amygdala
    Article Snippet: .. Sections were incubated for 15 min in 3% hydrogen peroxide to eliminate endogenous peroxidases, then rinsed and incubated with blocking buffer (5% bovine serum albumin, 0.3% Triton X-100) for 1 h. Sections were then incubated with a mouse anti-BrdU antibody (1∶1000; Sigma) overnight at 4°C, rinsed and transferred to biotinylated goat anti-mouse IgG (1∶200; Vector Laboratories) at room temperature for 2 h. BrdU was visualized using an avidin-biotin-horseradish peroxidase kit (Vector Laboratories) and reacted with diaminobenzidine (Sigma). ..

    Labeling:

    Article Title: Quantification and correlation of amyloid-β plaque load, glial activation, GABAergic interneuron numbers, and cognitive decline in the young TgF344-AD rat model of Alzheimer’s disease
    Article Snippet: .. After thorough rinsing, the sections were incubated with a corresponding biotinylated secondary antibody for 2 h and labeling was visualized using an avidin-biotin-horseradish peroxidase kit (1:200; Vectastaine Elite ABC Kit, Vector Laboratories), and developed with diaminobenzidine (1:200; DAB Peroxidase Substrate Kit, Vector Laboratories). ..



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